規(guī)格:型號齊全,*
價格:價優(yōu),歡迎詢價
產品簡介:
加壓毛細管電色譜是近年發(fā)展起來的一種新型微分離分析技術,它整合了毛細管電泳與液相色譜的優(yōu)點,
通過在填充有hplc填料的毛細管電色譜柱兩端施加高壓直流電場,
樣品在毛細管色譜柱中的保留行為同時受到電滲流及其在流動相與固定相之間分配系數的影響,大大提高了樣品分離能力。
序號 名稱 型號 說明
1 binary gradient syst triseptm 2100 pcec 配置詳見清單
2 isocratic system triseptm 2100 pcec 配置詳見清單
3 upgrader sys lc-pcec 配置詳見清單
產品屬性
型號:
輸出電流范圍:
精度:
rsd :
檢測限:
價格:
備注:
主要特點
加壓毛細管電色譜是近年發(fā)展起來的一種新型微分離分析技術,它整合了毛細管電泳與液相色譜的優(yōu)點,
通過在填充有hplc填料的毛細管電色譜柱兩端施加高壓直流電場,樣品在毛細管色譜柱中的保留行為同時受到電滲流及其在流動相與固定相之間分配系數的影響,
大大提高了樣品分離能力,在雙重分離機制的作用下, pcec 對于被分離樣品細微之處的分辨能力得到了*的提高。
代表了分析學界高效微分離的趨勢,尤其適用于復雜生物及化學體系的研究。 結合毛細管柱上檢測技術,
pcec 可與紫外檢測器( uv )、熒光檢測器(fld)、激光誘導熒光檢測器(lif)、電化學檢測器( ecd )及質譜( ms )等多種檢測手段聯用,
應用領域極為廣泛。
主要特點:
1、的分離分析性能
◆ 超高效分離:25萬以上理論塔板數/米
◆ 超快速分離:30秒鐘完成4種樣品的分離
◆ 超高峰容量:3倍于高效液相色譜的峰容量
◆ 超高精度分離:重現性與液相色譜媲美
2、雙重分離機理
◆ 具有液相色譜和電泳的雙重分離機理,使分離的選擇性得到*的提高!
3、一機三用
◆ *的模塊化設計,不同的組合方式即可實現加壓毛細管電色譜(pcec)、微徑液相色譜(μhplc)及毛細管電泳(ce)三種操作模式,
zui大限度發(fā)揮儀器潛能!
應用實例:
超高效分離: 25萬以上理論塔板數/米
圖一. pcec分離14種多環(huán)芳烴
zui高柱效可達25萬以上理論塔板數/米
超快速分離: 30秒鐘完成4種樣品的分離
圖二. pcec 與 capillary hplc之間的比較
pcec可在30秒鐘完成4種樣品的分離
超高峰容量: 3倍于高效液相色譜的峰容量
圖三. 加壓毛細管電色譜分析*解牛血清白蛋白
超高精度分離: 重現性可與液相色譜媲美
圖四. 連續(xù)10次進樣的保留時間(rt)與峰面積(pa)的重現性
圖五. 連續(xù)9次進樣的保留時間(rt)與峰面積(pa)的重現性
產品技術參數
triseptm- 2100pcec加壓毛細管電色譜由溶劑梯度洗脫系統(tǒng),納升級定量電動進樣閥、毛細管電色譜柱、uv/vis柱上檢測器(激光誘導熒光檢測器可選),
雙模式高壓電源和數據采集分析系統(tǒng)組成。
高壓*
item
specification
pump type
micro- volume double plunger pump (approx. 10ml per stroke)
pumping methods
constant flow delivery and constant pressure delivery
constant flow pumping
flow rate setting range
0.001-5ml/min [1.0-39.2mpa(10-400kgf/cm2)]
5.001-9.999ml/min [1.0-19.6mpa(10-200kgf/cm2)]
flow rate accuracy
±2% or ±2ml/min, whichever is greater(when water is pumped at 0.01-5ml/min, 1.0-39.2mpa(10-400kgf/cm2), with room temperature 20℃)
a different accuracy applies to low-pressure gradient delivery
flow rate precision
within ±0.3% (when water is pumped at 0.1-5ml/min, 1.0-39.2mpa(10-400kgf/cm2), with room temperature 20。c) )
constant pressure pumping
pressure setting range
1.0-39.2mpa(10-400kgf/cm2)[0.1mpa (1kgf/cm2)steps]
pressure accuracy
±10% or1.0mpa (10kgf/cm2), whichever is greater
pressure limits
upper and lower limits
damper
interior capacity 400ml approx
liquid-contacting part materials
sus316, ruby, sapphire, teflon ?, hasloyc?, peek, perfluoroelastomer zirconia, pctfe
suction filter
10μm mesh
line filter
5μm mesh, capacity 30ml approx.
time program
commands for flow rate, pressure, event functions, loop (for program repetition) 10files, 320 steps.
pressure display accuracy
±2% or 0.5mpa (5kgf/cm2), whichever is greater
plunger cleaning line
automatic rinsing possible with optional kit
size
w260×h 140 ×d 420mm, excluding projections
weight
11kg
operating temperature range
4-35。c
high-pressure gradient system specifications
number of solvents mixed
2
gradient profile
step and linear possible at multiple levels
maximum program steps
max.320 steps per program. 20program files
program duration
max.999.99 minutes (in 0.01minute steps
mix ratio setting range
0-* (in 0.1% steps)
concentration accuracy
±1% [with aqueous acetone/water, at 0-*, 0.1-3ml/min,1.0-39.2mpa(10-400kgf/cm2)]
flow rates poss inlet
0.001-9.999ml/min
檢測器
item
triseptm—2100 detector
light source
deuterium lamp
wavelength range
190-600nm cut-off filter to eliminate second order diffraction is automatically activated for the 371—600nm range.
spectral bandwidth
8nm
wavelength accuracy
1nm
wavelength reproducibility
0.1nm (*1)
drift
5′10-4 au/hour max. (250nm, room temperature constant, air in the cell) 6′10-4au/hour max. (250nm, room temperature fluctuation less than 2°c,
air in the cell)
noise level
3.5′10-6au
cell pressure tolerance
12mpa {120kgf/cm2}
dual wavelength mode (*3)
measurement wavelength
sampling frequency
sampling frequency
1.2 sec for one wavelength
response
se-lectable in 10 steps corresponding to time constant 0.05, 0.1, 0.5, 1.0, 1.5, 2.0, 3.0, 6.0, 8.0, 10.0 seconds
range
can be set between 0.0001 and 2.56 aufs in 0.0001 aufs steps
zero adjustment
auto zero function, baseline shift function
polarity switching wavelength scanning function
possible performs wavelength scanning with flow stopped. data stored in three files, one of which stores background.
background is subtracted from sample scans. file data is not saved up (lost when power is turned off).
wavelength steps
1-5nm, se-lectable in 5 steps, 2-5nm when w lamp is used. 10-50nm/sec, settable in 5 steps (according to the wavelength step) 1, 3, 10 nm/sec
scanning speed
spectrum plot output speed
ratio chromatogram
outputs the absorption ratio of two wavelengths
time program
available in detector or by system controller
set items
wavelength (including dual wavelengths), auto zero, range, marker, response, wavelength scanning, event, polarity, lamp on/off, loop
number of steps (detector program)
maximum 32 steps
output
output for recorder
10mv recorder terminals
output for integrator
6 steps: 0.5, 1, 2, 4, 1.25 and 2.5 au/v integrator output terminals output chromatogram in single wavelength mode or the ch1
chromatogram in dual wavelength mode. ratio chromatogram, spectrum, and ch2 chromatogram are output from the recorder output terminals.
lamp hour-meter
records up to 9999.9 hours
size
260(w) 140(h) 430(d) mm
weight
13 kg
operating temperature range
4°c—35°c
power supply
110-120v ac, 10% 150va 50-60hz
220-230v ac/240v ac, 10% 150va 50-60hz