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MAVEn™高通量16通道果蠅代謝監(jiān)測(cè)系統(tǒng)

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果蠅作為經(jīng)濟(jì)實(shí)用的模式動(dòng)物,可用于中樞神經(jīng)系統(tǒng)紊亂、炎癥性病變、心血管疾病、癌癥以及糖尿病等治療研究,而這些疾病的發(fā)生從生理上來(lái)說(shuō)都與生物個(gè)體*的代謝功能異常密切相關(guān)。

MAVEn™高通量16通道果蠅代謝監(jiān)測(cè)系統(tǒng)是由世界的美國(guó)Sable Systems International動(dòng)物代謝測(cè)量公司生產(chǎn)的一款16通道、高分辨率及自動(dòng)化的果蠅代謝監(jiān)測(cè)儀器,可廣泛用于代謝紊亂造成的各種流行疾病治療的機(jī)理研究。

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MAVEn™果蠅代謝系統(tǒng)作為果蠅代謝分型監(jiān)測(cè)方面的產(chǎn)品,主要具備以下特點(diǎn):

1. 改變了傳統(tǒng)的單只果蠅的封閉或半封閉式測(cè)量模式,實(shí)現(xiàn)每個(gè)測(cè)量室都有實(shí)時(shí)氣流通過(guò)的*開(kāi)放式測(cè)量,避免了測(cè)量時(shí)內(nèi)出現(xiàn)缺氧(hypoxia)或高碳酸血癥(hypercapnia),可一次測(cè)量多達(dá)16只個(gè)體。

2. 15秒就可以完成一只果蠅的代謝監(jiān)測(cè),這代表了目前技術(shù)的水平。

3. 數(shù)據(jù)可以通過(guò)SD卡把帶時(shí)間標(biāo)簽的CSV格式直接導(dǎo)出到電腦。

4. 可選配FLIC果蠅覓食、AD-2果蠅活動(dòng)、氣體(氧氣、二氧化碳、水汽以及其它可檢測(cè)氣體)等監(jiān)測(cè)單元。

5. 參考文獻(xiàn)多,高達(dá)4萬(wàn)多篇,屬于前沿科技。

image.png

具體性能指標(biāo):

1. 氣流流速:5毫升/分鐘-200毫升/分鐘,質(zhì)量流量計(jì),PID精確控制,精度為2%。

2. 昆蟲(chóng)測(cè)量時(shí)間:15秒-3小時(shí)可程序化選擇;基線測(cè)量時(shí)間:15秒-3小時(shí)可程序化選擇。

3. 氣壓測(cè)量:分辨率1Pa,精度0.05%。

4. 光照水平:0.1-5000勒克斯。

5. 溫度測(cè)量:0-50℃,分辨率0.01℃,精度±0.25℃。

6. 模擬輸入:6個(gè)模擬輸入,16bit分辨率,-5至+5伏電壓信號(hào),可接SSI其它儀器或?qū)嶒?yàn)室其它氣體分析儀等。

7. 數(shù)據(jù)格式:CSV格式;數(shù)據(jù)存儲(chǔ):SD卡,大支持32G的SD卡。

8. image.png雙通道高精度差分式氧氣分析測(cè)量?jī)x:測(cè)量技術(shù):燃料電池原理氧氣傳感器,雙通道;氧氣濃度量程0-*(用戶(hù)可自定義設(shè)置5個(gè)級(jí)別);差值量程±50%;精度0.1%(O2濃度2-*時(shí));分辨率0.0001%O2;漂移< 0.01%每小時(shí)(溫度恒定情況下);響應(yīng)時(shí)間小于7秒;24小時(shí)漂移<0.01%;20分鐘噪音<3ppm RMS;數(shù)字過(guò)濾(噪音)0-40秒可調(diào),增幅0.2秒,內(nèi)置A/D轉(zhuǎn)換器分辨率16bits;溫度、壓力補(bǔ)償;傳感器溫度測(cè)量范圍0-60℃,精度0.2℃,分辨率0.001℃;大氣壓測(cè)量分辨率0.0001kPa,精度為滿(mǎn)量程的0.05%;適用流量范圍5-2000mL/min;4通道模擬信號(hào)輸出(0-5V BNC)可輸出通道1的氧氣濃度,通道2的氧氣濃度,1和2的差值,大氣壓;數(shù)字輸出:RS-232;具4行文字LCD顯示屏,帶背光,可同時(shí)顯示2個(gè)通道的氧氣含量和它們的差值,以及大氣壓;*PID(Proportional-Integral-Derivative)溫控單元,保證內(nèi)部氧氣傳感器溫度恒定,進(jìn)一步提高了氧氣測(cè)量的精度和穩(wěn)定性;供電12-24VDC,8A,配交流電適配器;工作溫度:5-45℃,無(wú)冷凝;重量6.4kg;尺寸43.2cm×35.6cm×20.3cm

9. 超高精度二氧化碳分析測(cè)量?jī)x:用于測(cè)量微小昆蟲(chóng)(比如果蠅、蚊子等)或蜱螨類(lèi)微小動(dòng)物的呼吸代謝,可同時(shí)測(cè)量CO2濃度和H2O濃度;CO2量程0-3000ppm;準(zhǔn)確度<1%;分辨率0.01ppm;H2O量程0-60mmol/mol;準(zhǔn)確度1%;

10. 二次抽樣單元:內(nèi)置氣泵、精密針閥、質(zhì)量流量計(jì),可用來(lái)給氣流樣本做二次抽樣,也可單獨(dú)作為氣源使用;流量范圍5-2000mL/min;精度為讀數(shù)的10%;分辨率1mL/min;具備2行顯示LCD顯示屏;帶0-5V BNC模擬信號(hào)輸出;數(shù)字輸出RS-232;供電12-15VDC,20-350mA,配交流電適配器;工作溫度:0-50℃,無(wú)冷凝;重量1.5kg;尺寸16cm×13cm×20cm;

產(chǎn)地:美國(guó)

文獻(xiàn)案例:

在2016年已發(fā)表的果蠅有關(guān)文獻(xiàn)中,使用SSI果蠅代謝監(jiān)測(cè)系統(tǒng)的達(dá)14篇,2015年11篇,截止目前相關(guān)文獻(xiàn)共計(jì)500多篇。

1. Andrew N R, Ghaedi B, Groenewald B. The role of nest surface temperatures and the brain in influencing ant metabolic rates[J]. Journal of Thermal Biology, 2016, 60: 132-139.

2. Baaren J, Dufour C M S, Pierre J S, et al. Evolution of life‐history traits and mating strategy in males: a case study on two populations of a Drosophila parasitoid[J]. Biological Journal of the Linnean Society, 2016, 117(2): 231-240.

3. Bartholomew N R, Burdett J M, VandenBrooks J M, et al. Impaired climbing and flight behaviour in Drosophila melanogaster following carbon dioxide anaesthesia[J]. Scientific reports, 2015, 5.

4. Basson C H, Clusella-Trullas S. The behavior-physiology nexus: behavioral and physiological compensation are relied on to different extents between seasons[J]. Physiological and Biochemical Zoology, 2015, 88(4): 384-394.

5. Bosco G, Clamer M, Messulam E, et al. EFFECTS OF OXYGEN CONCENTRATION AND PRESSURE ON Drosophila melanogaster: OXIDATIVE STRESS, MITOCHONDRIAL ACTIVITY, AND SURVIVORSHIP[J]. Archives of insect biochemistry and physiology, 2015, 88(4): 222-234.

6. Casas J, Body M, Gutzwiller F, et al. Increasing metabolic rate despite declining body weight in an adult parasitoid wasp[J]. Journal of insect physiology, 2015, 79: 27-35.

7. Correa Y D C G, Faroni L R A, Haddi K, et al. Locomotory and physiological responses induced by clove and cinnamon essential oils in the maize weevil Sitophilus zeamais[J]. Pesticide biochemistry and physiology, 2015, 125: 31-37.

8. DeVries Z C, Kells S A, Appel A G. Estimating the critical thermal maximum (CT max) of bed bugs, Cimex lectularius: Comparing thermolimit respirometry with traditional visual methods[J]. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 2016, 197: 52-57.

9. Dreiss A N, Séchaud R, Béziers P, et al. Social huddling and physiological thermoregulation are related to melanism in the nocturnal barn owl[J]. Oecologia, 2016, 180(2): 371-381.

10. Duun Rohde P, Krag K, Loeschcke V, et al. A Quantitative Genomic Approach for Analysis of Fitness and Stress Related Traits in a Drosophila melanogaster Model Population[J]. International Journal of Genomics, 2016, 2016.

11. Fischer K E, Gelfond J A L, Soto V Y, et al. Health effects of long-term rapamycin treatment: the impact on mouse health of enteric rapamycin treatment from four months of age throughout life[J]. PloS one, 2015, 10(5): e0126644.

12. Groom D J E, Toledo M C B, Welch K C. Wingbeat kinematics and energetics during weightlifting in hovering hummingbirds across an elevational gradient[J]. Journal of Comparative Physiology B, 2016: 1-18.

13. Gudowska A, Boardman L, Terblanche J S. The closed spiracle phase of discontinuous gas exchange predicts diving duration in the grasshopper, Paracinema tricolor[J]. Journal of Experimental Biology, 2016: jeb. 135129.

14. Haddi K, Mendes M V, Barcellos M S, et al. Sexual Success after Stress? Imidacloprid-Induced Hormesis in Males of the Neotropical Stink Bug Euschistus heros[J]. PloS one, 2016, 11(6): e0156616.

15. Haddi K, Oliveira E E, Faroni L R A, et al. Sublethal exposure to clove and cinnamon essential oils induces hormetic-like responses and disturbs behavioral and respiratory responses in Sitophilus zeamais (Coleoptera: Curculionidae)[J]. Journal of economic entomology, 2015: tov255.

16. Horváthová T, Antol A, Czarnoleski M, et al. Does temperature and oxygen affect duration of intramarsupial development and juvenile growth in the terrestrial isopod Porcellio scaber (Crustacea, Malacostraca)?[J]. ZooKeys, 2015 (515): 67.

17. Kivel? S M, Lehmann P, Gotthard K. Do respiratory limitations affect metabolism of insect larvae before moulting: an empirical test at the individual level[J]. Journal of Experimental Biology, 2016: jeb. 140442.

18. Lebeau J, Wesselingh R A, Van Dyck H. Nectar resource limitation affects butterfly flight performance and metabolism differently in intensive and extensive agricultural landscapes[C]//Proc. R. Soc. B. The Royal Society, 2016, 283(1830): .

19. MacMillan H A, Schou M F, Kristensen T N, et al. Preservation of potassium balance is strongly associated with insect cold tolerance in the field: a seasonal study of Drosophila subobscura[J]. Biology letters, 2016, 12(5): .

20. Meyers P J, Powell T H Q, Walden K K O, et al. Divergence of the diapause transcriptome in apple maggot flies: winter regulation and post-winter transcriptional repression[J]. Journal of Experimental Biology, 2016: jeb. 140566.

21. Plav?in I, Sta?ková T, ?ery M, et al. Hormonal enhancement of insecticide efficacy in Tribolium castaneum: Oxidative stress and metabolic aspects[J]. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2015, 170: 19-27.

22. Rodrigues C G, Krüger A P, Barbosa W F, et al. Leaf Fertilizers Affect Survival and Behavior of the Neotropical Stingless Bee Friesella schrottkyi (Meliponini: Apidae: Hymenoptera)[J]. Journal of economic entomology, 2016, 109(3): 1001-1008.

23. Thienel M, Canals M, Bozinovic F, et al. The effects of temperature on the gas exchange cycle in Agathemera crassa[J]. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 2015, 183: 126-130.

24. Williams C M, Chick W D, Sinclair B J. A cross‐seasonal perspective on local adaptation: metabolic plasticity mediates responses to winter in a thermal‐generalist moth[J]. Functional Ecology, 2015, 29(4): 549-561.

25. Williams C M, Szejner-Sigal A, Morgan T J, et al. Adaptation to Low Temperature Exposure Increases Metabolic Rates Independently of Growth Rates[J]. Integrative and comparative biology, 2016: icw009.



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