The Cayman Chemical Protein Determination Kit is a microplate based, colorimetric method for rapid total protein quantification. Based on the well-known Bradford method,1↗ it takes advantage of the color change of Coomassie® Dye when it binds to proteins in acidic medium. When the dye binds, there is an immediate shift of the absorption maximum from 465 to 595 nm with a simultaneous change in color from brown to blue.
1 Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72 248-254 （1976）.

 

 High-Binding 96-Well Solid Plate   
96-Well Cover Sheet   
Protein Determination BSA Standard  1 ea 
Protein Determination Assay Reagent